rabbit polyclonal anti pegfr tyr1173 antibody  (Cell Signaling Technology Inc)

Journal: The EMBO Journal

Article Title: Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputs

doi: 10.15252/embj.2020107182

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Article Snippet: Rabbit polyclonal pEGFR Thr669 , Cell Signaling Technology , 3056s.

Techniques: Virus, Recombinant, Extraction, Multiplex sample analysis, Transfection, Molecular Weight, Marker, Reverse Transcription, Protease Inhibitor, Membrane, Cell Culture, Clinical Proteomics, Spectroscopy, Imaging, cDNA Synthesis, Plasmid Preparation, Negative Control, Mutagenesis, Clone Assay, Generated, Software, Microscopy

Journal: Cancers

Article Title: EGFRvIII Promotes Cell Survival during Endoplasmic Reticulum Stress through a Reticulocalbin 1-Dependent Mechanism

doi: 10.3390/cancers13061198

Figure Lengend Snippet: EGFRvIII expression correlates with RCN1 expression in glioblastoma cell lines. ( A , B ). A series of EGFRvIII positive and negative glioblastoma cell lines were lysed and assessed for EGFRvIII, RCN1 and GAPDH expression by Western blot. ( C , D ) The same cell lines as above were assessed for RCN1 gene expression by qPCR. Three cell lines with variant sub-populations with differing EGFR expression were ( E ) lysed and assessed for EGFR, RCN1 and GAPDH expression by Western blot and ( F ) RCN1 gene expression by qPCR. ( G ) The relationship between high (Red) and low (Green) EGFR and RCN1 gene expression with patient survival was determined through mining a SurvExpress TCGA dataset. Kaplan–Meier survival curves were evaluated from the TCGA, n = 148. * p < 0.05; *** p < 0.001.

Article Snippet: The rabbit polyclonal antibodies directed against pEGFR, EGFR, pEIF2α, EIF2α and GAPDH and the mouse monoclonal antibody directed against ATF4 were all obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Expressing, Western Blot, Gene Expression, Variant Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Type 2 diabetes mellitus facilitates endometrial hyperplasia progression by activating the proliferative function of mucin O-glycosylating enzyme GALNT2.

doi: 10.1016/j.biopha.2020.110764

Figure Lengend Snippet: Fig. 4. GALNT2 disturbance facilitates proliferation and endometrial hyperplasia progression by modifying phosphorylation and activity of EGFR. A: Band diagram and statistical map of EGFR、pEGFR、AKT、pAKT、ERK and pERK protein expressions in rat uterus. B: Band diagram and statistical map of GALNT2 protein expression in Ishikawa cells after transfection and high sugar stimulation (n = 3). C: The proliferation of Ishikawa cells after transfection and high sugar stimulation (n = 3). D: Band diagram and statistical map of pAKT, pERK and pEGFR protein expressions in Ishikawa cells after transfection and high sugar stimulation (n = 3). Data are expressed as Mean ± SEM, n = 6, *P < 0.05, **P < 0.01, compared to the N group; ##P < 0.01, compared to the NH group; %P < 0.05, %%P < 0.01, compared to the MOCK-N group; @@P < 0.01, compared to the si-GALNT2-N group.

Article Snippet: Rabbit polyclonal antibody for pEGFR (3777) was from CST, Inc. (Boston, United States).

Techniques: Phospho-proteomics, Activity Assay, Expressing, Transfection